Fluorescein isothiocyanate stability in different solvents
نویسندگان
چکیده
Well-established label in biomolecules analysis, fluorescein isothiocyanate is commercially available its two different isomers with equal fluorescence sensitivity, differing meta- vs. para-isothiocyanate position. It was found this study that the stability of depends on polarity solvents and pyridine (stabiliser) presence. The lowest has water solvent. In acidic solution, their quinoid (acid) form switches to lactone form. p-quinoid higher tendency create dimers trimers less soluble acetone than
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ژورنال
عنوان ژورنال: Monatshefte Fur Chemie
سال: 2021
ISSN: ['1434-4475', '0026-9247']
DOI: https://doi.org/10.1007/s00706-021-02852-1